Part:BBa_K511823

Inducible Red Fluorescent Protein Generator (UAS-Gal4-mKate) MammoBlock Device

This MammoBlock composite device produces the monomeric red fluorescent protein mKate when induced with Gal4 transactivator variants and otherwise produces mKate at a low, basal (OFF) level.

Another activator promoter pair we make use of is the Gal4-UAS system. Gal4VP16 is an activator capable of binding to UAS promoter site and activating expression of downstream genes. Here we characterize this interaction with a set of transfections. Cells were transfected with UAS:(mKate, eYFP-FF4, eBFP2, or H2B-citrine) and Hef1a:GV16. Controls lack the Hef1a:GV16 plasmid. The above graph displays mean fluoresence in the denoted channels.

As can be clearly seen, Hef1a:GV16 results in significant activation of the UAS promoter. This results in the observed increases in mean fluoresence compared to control populations. With the exception of UAS:eBFP2, we see more than 20-fold increase in mean fluorescence.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 126
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 126
  Illegal NheI site found at 154
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 126
  Illegal XhoI site found at 83
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 126
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 126
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 876
  Illegal SapI.rc site found at 258